[HTML][HTML] Conjugation of Nedd8 to CUL1 enhances the ability of the ROC1-CUL1 complex to promote ubiquitin polymerization
The SCF-ROC1 ubiquitin-protein isopeptide ligase (E3) ubiquitin ligase complex targets the
ubiquitination and subsequent degradation of protein substrates required for the regulation
of cell cycle progression and signal transduction pathways. We have previously shown that
ROC1-CUL1 is a core subassembly within the SCF-ROC1 complex, capable of supporting
the polymerization of ubiquitin. This report describes that the CUL1 subunit of the bacterially
expressed, unmodified ROC1-CUL1 complex is conjugated with Nedd8 at Lys-720 by HeLa …
ubiquitination and subsequent degradation of protein substrates required for the regulation
of cell cycle progression and signal transduction pathways. We have previously shown that
ROC1-CUL1 is a core subassembly within the SCF-ROC1 complex, capable of supporting
the polymerization of ubiquitin. This report describes that the CUL1 subunit of the bacterially
expressed, unmodified ROC1-CUL1 complex is conjugated with Nedd8 at Lys-720 by HeLa …