The effects of anesthesia, mouse strain and age on intraocular pressure and an improved murine model of experimental glaucoma
FE Cone, MR Steinhart, EN Oglesby… - Experimental eye …, 2012 - Elsevier
The purpose of this study was to improve a mouse model of chronic intraocular pressure
(IOP) elevation utilizing microbead injection in two strains of mice and to assess the effect of
age and anesthesia on measured IOP. We compared our previous model with two modified
protocols for injecting polystyrene microbeads and viscoelastic material in CD1or C57BL/6
mice. The measured outcomes were degree of IOP elevation and production of axonal loss.
The first new protocol was injection of 3μL of equal volumes of 6μm and 1μm diameter …
(IOP) elevation utilizing microbead injection in two strains of mice and to assess the effect of
age and anesthesia on measured IOP. We compared our previous model with two modified
protocols for injecting polystyrene microbeads and viscoelastic material in CD1or C57BL/6
mice. The measured outcomes were degree of IOP elevation and production of axonal loss.
The first new protocol was injection of 3μL of equal volumes of 6μm and 1μm diameter …