[HTML][HTML] The Ddx20/DP103 dead box protein represses transcriptional activation by Egr2/Krox-20
AL Gillian, J Svaren - Journal of Biological Chemistry, 2004 - ASBMB
The early growth response 2 (Egr2/Krox-20) transcription factor is essential for myelination
of the peripheral nervous system and segmentation of the vertebrate hindbrain. To probe the
mechanism by which Egr2 is regulated, we used a yeast two-hybrid assay and identified an
RNA helicase, Ddx20 (DP103/Gemin3), as an Egr2-interacting protein. Mammalian two-
hybrid assays indicated that Ddx20 can interact with Egr1, Egr3, and Egr4, in addition to
Egr2, making it the only known cofactor that interacts with all four Egr family members. Using …
of the peripheral nervous system and segmentation of the vertebrate hindbrain. To probe the
mechanism by which Egr2 is regulated, we used a yeast two-hybrid assay and identified an
RNA helicase, Ddx20 (DP103/Gemin3), as an Egr2-interacting protein. Mammalian two-
hybrid assays indicated that Ddx20 can interact with Egr1, Egr3, and Egr4, in addition to
Egr2, making it the only known cofactor that interacts with all four Egr family members. Using …