A rapid method for in situ hybridization for viral DNA in brain biopsies from patients with AIDS
SA Houff, D Katz, CV Kufta, EO Major - Aids, 1989 - journals.lww.com
SA Houff, D Katz, CV Kufta, EO Major
Aids, 1989•journals.lww.comBrain biopsy is often necessary in the diagnosis of neurological complications found in AIDS
patients. We describe here a rapid method of tissue preparation and in situ DNA
hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the
diagnosis of progressive multifocal leukoencephalopathy to be established on the day of
surgery. Once the diagnosis is established, therapeutic and management decisions can be
made more easily. The commercial availability of biotinylated probes for several of the DNA …
patients. We describe here a rapid method of tissue preparation and in situ DNA
hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the
diagnosis of progressive multifocal leukoencephalopathy to be established on the day of
surgery. Once the diagnosis is established, therapeutic and management decisions can be
made more easily. The commercial availability of biotinylated probes for several of the DNA …
Abstract
Brain biopsy is often necessary in the diagnosis of neurological complications found in AIDS patients. We describe here a rapid method of tissue preparation and in situ DNA hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the diagnosis of progressive multifocal leukoencephalopathy to be established on the day of surgery. Once the diagnosis is established, therapeutic and management decisions can be made more easily. The commercial availability of biotinylated probes for several of the DNA viruses most frequently encountered in brain infections of AIDS patients will provide wide application of these techniques to patient management.
Lippincott Williams & Wilkins