[HTML][HTML] Purification and Characterization of Human NTH1, a Homolog ofEscherichia coli Endonuclease III: DIRECT IDENTIFICATION OF LYS-212 AS THE ACTIVE …
The human endonuclease III (hNTH1), a homolog of the Escherichia coli enzyme (Nth), is a
DNA glycosylase with abasic (apurinic/apyrimidinic (AP)) lyase activity and specifically
cleaves oxidatively damaged pyrimidines in DNA. Its cDNA was cloned, and the full-length
enzyme (304 amino acid residues) was expressed as a glutathione S-transferase fusion
polypeptide in E. coli. Purified wild-type protein with two additional amino acid residues and
a truncated protein with deletion of 22 residues at the NH 2 terminus were equally active and …
DNA glycosylase with abasic (apurinic/apyrimidinic (AP)) lyase activity and specifically
cleaves oxidatively damaged pyrimidines in DNA. Its cDNA was cloned, and the full-length
enzyme (304 amino acid residues) was expressed as a glutathione S-transferase fusion
polypeptide in E. coli. Purified wild-type protein with two additional amino acid residues and
a truncated protein with deletion of 22 residues at the NH 2 terminus were equally active and …