Expression and purification of active mouse and human NEIL3 proteins
M Liu, V Bandaru, A Holmes, AM Averill… - Protein expression and …, 2012 - Elsevier
Endonuclease VIII-like 3 (Neil3) is one of the five DNA glycosylases found in mammals that
recognize and remove oxidized bases, and initiate the base excision repair (BER) pathway.
Previous attempts to express and purify the mouse and human orthologs of Neil3 in their
active form have not been successful. Here we report the construction of bicistronic
expression vectors for expressing in Escherichia coli the full-length mouse Neil3
(MmuNeil3), its glycosylase domain (MmuNeil3Δ324), as well as the glycosylase domain of …
recognize and remove oxidized bases, and initiate the base excision repair (BER) pathway.
Previous attempts to express and purify the mouse and human orthologs of Neil3 in their
active form have not been successful. Here we report the construction of bicistronic
expression vectors for expressing in Escherichia coli the full-length mouse Neil3
(MmuNeil3), its glycosylase domain (MmuNeil3Δ324), as well as the glycosylase domain of …