[HTML][HTML] Signaling via the T cell antigen receptor induces phosphorylation of Stat1 on serine 727
AM Gamero, AC Larner - Journal of Biological Chemistry, 2000 - ASBMB
The Stat1 transcription factor plays a pivotal role in both, the antiviral and antigrowth actions
of interferons. Stat1 acquires the ability to bind DNA by becoming phosphorylated on Tyr
701. However, to effectively stimulate gene transcription, it must also be phosphorylated on
Ser 727. We show that engagement of T cell antigen receptor (TCR)/CD3 complex in either
Jurkat cells or peripheral blood lymphocytes stimulates phosphorylation of Ser 727 but not
Tyr 701 of Stat1. This process does not require the expression of tyrosine kinases Lck and …
of interferons. Stat1 acquires the ability to bind DNA by becoming phosphorylated on Tyr
701. However, to effectively stimulate gene transcription, it must also be phosphorylated on
Ser 727. We show that engagement of T cell antigen receptor (TCR)/CD3 complex in either
Jurkat cells or peripheral blood lymphocytes stimulates phosphorylation of Ser 727 but not
Tyr 701 of Stat1. This process does not require the expression of tyrosine kinases Lck and …